Computational Biology Unit

Computational Biology Unit

Paolo Provero

We're an interdisciplinary reseach team interested in diverse topics regarding computational biology and bioinformatics. Although we have started our collaboration already years ago, the CBU was officially formed in 2006 when the Molecular Biotechnology Center was inaugurated to pool the University's diverse biotechnology research groups and form a center of excellence for interdisciplinary teaching.

The research activity of the Computational Biology Unit focuses on gene regulation at the transcriptional and post-transcriptional levels. In particular we are currently investigating:

  • The function, evolution and variation of regulatory regions in the human genome, with an approach based on Total Binding Affinity as a predictor of the binding of transcription factors to cis-regulatory regions. This approach naturally takes into account the contribution of weak binding sites and, from an evolutionary perspective, the phenomenon of binding site turnover. We are applying this approach to (a) the prediction of gene expression from sequence and chromatin data in human cell lines (b) the study of naturally occurring variation in gene expression in human populations, and the unraveling of its genetic origin (c) the evolution of cis-regulatory regions as a major determinant of anatomical and behavioral differences in humans and great apes

  • The role of 3' UTRs in the post-transcriptional regulation of gene expression, in two related aspects: (a) competing endogenous RNAs, where the titration of microRNA abundance by their targets results in a regulatory cross-talk by targets of the same microRNAs that is wholly independent of their gene products (b) shortening of 3' UTR, in which proliferating cells escape regulation by microRNAs and other RNA-binding trans-acting factors by expressing shortened 3' UTR isoforms lacking binding sites for those factors.

The unit also collaborates with several experimental laboratories, within the MBC and elsewhere, providing computational and data-mining expertise.

Please visit our web site to access our web resources

Unit members

Paolo Prof. PROVERO - Associate Professor
Ugo ALA – Post doctoral fellow
Ivan Molineris – Post doctoral fellow
Elena Grassi -  PhD Student
Davide Marnetto – PhD Student